SRL

Pack Size

Price

100 Gms
13.16
500 Gms
55.27

Prices are indicative and are subject to change

Appearance (Colour)

Light tan

Appearance (Form)

Free flowing, homogeneous powder

Solubility

65.00 gm/liter

Solubility before autoclaving (Clarity)

Clear to very slightly opalescent

Gel strength (1.5% gel)

Firm, comparable with 1.5% Agar gel

pH (25°C)

7.2 ± 0.2

Prepared Medium Appearance after autoclaving (Clarity)

Opaque

Prepared Medium Appearance after autoclaving (Colour)

Yellow

Cultural Response

Inoculate and incubate at 37°C for 24-48 hours

Organism

Staphylococcus aureus ATCC 25923

Inoculum (cfu) 10-100

Growth : Good, Recovery Rate : >=50%, Colony colour : Grey -Black, Lecithinase halo : (+)

Organism

Staphylococcus aureus ATCC 6538

Inoculum (cfu) 10-100

Growth : Good, Recovery Rate : >=50%, Colony colour : Grey -Black, Lecithinase halo : (+)

Organism

Proteus mirabilis ATCC 25933

Inoculum (cfu) 10-100

Growth : Good, Recovery Rate : >=50%, Colony colour : Brown - Black, Lecithinase halo : (-)

Organism

Staphylococcus epidermidis ATCC 12228

Inoculum (cfu) 10-100

Growth : Poor - good, Recovery Rate : 30 - 40%, Colony colour : Black, Lecithinase halo : (-)

Organism

Bacillus subtilis ATCC 6633

Inoculum (cfu) 10-100

Growth : Poor- fair, Recovery Rate : 0 -10%, Colony colour : Brown -Black, Lecithinase halo : (-)

Organism

Escherichia coli ATCC 25922

Inoculum (cfu) 10-100

Growth : None - poor, Recovery Rate : 0 - 10%, Colony colour : Brown black, Lecithinase halo : (-)

Organism

Micrococcus luteus ATCC 10240

Inoculum (cfu) 10-100

Growth : None -poor, Recovery Rate : 30 - 40%, Colony colour : Very small shades of black -brown, Lecithinase halo : (-)

Key

Lecithinase halo : (+) indicates opaque zone around colony
Lecithinase halo : (-) indicates no opaque zone around colony

Applications :


Recommended medium for isolation and enumeration of coagulase positive staphylococci from food and other specimens.

Composition :


Ingredients

gm/lt.

Agar

22.00

L-Glycine

12.00

Sodium pyruvate

10.00

Pancreatic digest of casein

10.00

Meat extract

5.00

Lithium chloride

5.00

Yeast extract

1.00

Directions :


1. Add 65.00 gm powder to 940.0 ml distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4. Cool to 45-50°C.
5. Add aseptically 10 ml sterile 1% Potassium tellurite solution (91488) and mix well.
6. Add 50 ml of sterile egg yolk emulsion.
7. Mix well.

Storage

8 to 25°C (Cool & Dry Area)

Shelf Life

36 Months

IMDG Identification

Not Regulated for Transport (Non-Haz)

HSN Code :


100 Gms

38210000 (GST 18%)

500 Gms

38210000 (GST 18%)

Type of Packing :


100 Gms

Plastic Bottle

500 Gms

Plastic Bottle

Safety Datasheet

Product Datasheet

Sample COA

Certificate Of Analysis

GEM Catalogue ID

5116877-87400552489 GeM link