Appearance (Colour)
Light tan
Appearance (Form)
Free flowing, homogeneous powder
Solubility
65.00 gm/liter
Solubility before autoclaving (Clarity)
Clear to very slightly opalescent
Gel strength (1.5% gel)
Firm, comparable with 1.5% Agar gel
pH (25°C)
7.2 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Opaque
Prepared Medium Appearance after autoclaving (Colour)
Yellow
Cultural Response
Inoculate and incubate at 37°C for 24-48 hours
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good, Recovery Rate : >=50%, Colony colour : Grey -Black, Lecithinase halo : (+)
Organism
Staphylococcus aureus ATCC 6538
Inoculum (cfu) 10-100
Growth : Good, Recovery Rate : >=50%, Colony colour : Grey -Black, Lecithinase halo : (+)
Organism
Proteus mirabilis ATCC 25933
Inoculum (cfu) 10-100
Growth : Good, Recovery Rate : >=50%, Colony colour : Brown - Black, Lecithinase halo : (-)
Organism
Staphylococcus epidermidis ATCC 12228
Inoculum (cfu) 10-100
Growth : Poor - good, Recovery Rate : 30 - 40%, Colony colour : Black, Lecithinase halo : (-)
Organism
Bacillus subtilis ATCC 6633
Inoculum (cfu) 10-100
Growth : Poor- fair, Recovery Rate : 0 -10%, Colony colour : Brown -Black, Lecithinase halo : (-)
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : None - poor, Recovery Rate : 0 - 10%, Colony colour : Brown black, Lecithinase halo : (-)
Organism
Micrococcus luteus ATCC 10240
Inoculum (cfu) 10-100
Growth : None -poor, Recovery Rate : 30 - 40%, Colony colour : Very small shades of black -brown, Lecithinase halo : (-)
Key
Lecithinase halo : (+) indicates opaque zone around colony
Lecithinase halo : (-) indicates no opaque zone around colony
Applications
Recommended medium for isolation and enumeration of coagulase positive staphylococci from food and other specimens.
Composition
Ingredients
gm/lt.
Agar
22.00
L-Glycine
12.00
Sodium pyruvate
10.00
Pancreatic digest of casein
10.00
Meat extract
5.00
Lithium chloride
5.00
Yeast extract
1.00
Directions
1. Add 65.00 gm powder to 940.0 ml distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4. Cool to 45-50°C.
5. Add aseptically 10 ml sterile 1% Potassium tellurite solution (91488) and mix well.
6. Add 50 ml of sterile egg yolk emulsion.
7. Mix well.
