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Light tan
Free flowing, homogeneous powder
65.00 gms/liter
Clear to very slightly opalescent
Firm, comparable with 2.0% agar gel
7.00 - 7.40
Yellow
Opaque
Inoculate and incubate at 35 -37°C for 24-48 hours with added supplements and Egg yolk emulsion.
Staphylococcus aureus ATCC 25923
Growth : Good
Recovery Rate : >=50.0%
Colony colour : Grey -Black
Lecithinase activity : positive,clear zone around the colony
Staphylococcus aureus ATCC 6538
Growth : Good
Recovery Rate : >=50.0%
Colony colour : Grey -Black
Lecithinase activity : positive,clear zone around the colony
Proteus mirabilis ATCC 25933
Growth : Good
Recovery Rate : >=50.0%
Colony colour : Brown - Black,
Lecithinase activity :negative, no clear zone around the colony
Staphylococcus epidermidis ATCC 12228
Growth : Poor - good
Recovery Rate : 30 - 40%
Colony colour : Black
Lecithinase activity : negative,no clear zone around the colony
Bacillus subtilis ATCC 6633
Growth : Poor- fair
Recovery Rate : 0 -10%
Colony colour : Brown -Black
Lecithinase activity : negative,no clear zone around the colony
Escherichia coli ATCC 25922
Growth : None - poor
Recovery Rate : 0 - 10%
Colony colour : Brown black
Lecithinase activity : negative,no clear zone around the colony
Micrococcus luteus ATCC 10240
Growth : None -poor
Recovery Rate : 30 - 40%
Colony colour : very small shades of black-brown
Lecithinase activity : negative, no clear zone around the colony
Recommended medium for isolation and enumeration of coagulase positive staphylococci from food and other specimens.This medium is in accordance to specifications given by BIS under IS : 5887 (Part II)1976.
gms/lt.
10.00
5.00
1.00
12.00
12.00
5.00
20.00
1. Add 65.00 gm powder to 940.0 ml distilled/deionized water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4. Cool to 45-50°C.
5. Add aseptically 10 ml sterile 1% Potassium tellurite solution (91488) and mix well.
6. Add 50 ml of sterile egg yolk emulsion.
7. Mix well.
8 to 25°C (Cool & Dry Area)
36 Months
Not Regulated for Transport (Non-Haz)
38210000 (GST 18%)
38210000 (GST 18%)
Plastic Bottle
Plastic Bottle
