Pack Size

Price

100 Gms
13.16
500 Gms
55.27

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Appearance (Colour)

Light tan

Appearance (Form)

Free flowing, homogeneous powder

Solubility

65.00 gm/liter

Solubility before autoclaving (Clarity)

Clear to very slightly opalescent

Gel strength (1.5% gel)

Firm, comparable with 1.5% Agar gel

pH (25°C)

7.2 ± 0.2

Prepared Medium Appearance after autoclaving (Clarity)

Opaque

Prepared Medium Appearance after autoclaving (Colour)

Yellow

Cultural Response

Inoculate and incubate at 37°C for 24-48 hours

Organism

Staphylococcus aureus ATCC 25923

Inoculum (cfu) 10-100

Growth : Good, Recovery Rate : >=50%, Colony colour : Grey -Black, Lecithinase halo : (+)

Organism

Staphylococcus aureus ATCC 6538

Inoculum (cfu) 10-100

Growth : Good, Recovery Rate : >=50%, Colony colour : Grey -Black, Lecithinase halo : (+)

Organism

Proteus mirabilis ATCC 25933

Inoculum (cfu) 10-100

Growth : Good, Recovery Rate : >=50%, Colony colour : Brown - Black, Lecithinase halo : (-)

Organism

Staphylococcus epidermidis ATCC 12228

Inoculum (cfu) 10-100

Growth : Poor - good, Recovery Rate : 30 - 40%, Colony colour : Black, Lecithinase halo : (-)

Organism

Bacillus subtilis ATCC 6633

Inoculum (cfu) 10-100

Growth : Poor- fair, Recovery Rate : 0 -10%, Colony colour : Brown -Black, Lecithinase halo : (-)

Organism

Escherichia coli ATCC 25922

Inoculum (cfu) 10-100

Growth : None - poor, Recovery Rate : 0 - 10%, Colony colour : Brown black, Lecithinase halo : (-)

Organism

Micrococcus luteus ATCC 10240

Inoculum (cfu) 10-100

Growth : None -poor, Recovery Rate : 30 - 40%, Colony colour : Very small shades of black -brown, Lecithinase halo : (-)

Key

Lecithinase halo : (+) indicates opaque zone around colony
Lecithinase halo : (-) indicates no opaque zone around colony

Applications :


Recommended medium for isolation and enumeration of coagulase positive staphylococci from food and other specimens.

Composition :


Ingredients

gm/lt.

Agar

22.00

L-Glycine

12.00

Sodium pyruvate

10.00

Pancreatic digest of casein

10.00

Meat extract

5.00

Lithium chloride

5.00

Yeast extract

1.00

Directions :


1. Add 65.00 gm powder to 940.0 ml distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4. Cool to 45-50°C.
5. Add aseptically 10 ml sterile 1% Potassium tellurite solution (91488) and mix well.
6. Add 50 ml of sterile egg yolk emulsion.
7. Mix well.

Storage

8 to 25°C (Cool & Dry Area)

Shelf Life

36 Months

IMDG Identification

Not Regulated for Transport (Non-Haz)

HSN Code :


100 Gms

38210000 (GST 18%)

500 Gms

38210000 (GST 18%)

Type of Packing :


100 Gms

Plastic Bottle

500 Gms

Plastic Bottle

Safety Datasheet

Product Datasheet

Sample COA

Certificate Of Analysis

GEM Catalogue ID

5116877-87400552489 GeM link