Pack Size

Price

100 Gms
19.74
500 Gms
69.75

Prices are indicative and are subject to change

Appearance (Colour)

Cream to yellow coloured

Appearance (Form)

Free flowing, homogeneous powder

Solubility before autoclaving (Clarity)

Clear to very slightly opalescent

Solubility

63.05 gm/liter

Gel strength (1.5% gel)

Firm, comparable with 1.5% Agar gel

pH (25°C)

7.0 ± 0.2

Prepared Medium Appearance after autoclaving (Clarity)

Clear to very slightly opalescent

Prepared Medium Appearance after autoclaving (Colour)

Light to medium amber

Cultural Response

Inoculate and incubate at 35°C - 37°C for 24-48 hours

Organism

Bacillus subtilis ATCC 6633

Inoculum (cfu) 10-100

Growth : None- poor, Recovery : <=10%, Colony colour : Dark Brown Matt, Lecithinase : (-)

Organism

Staphylococcus aureus ATCC 25923

Inoculum (cfu) 10-100

Growth : Good, Recovery : =>50%, Colony colour : Grey - Black shiny, Lecithinase : (+)

Organism

Proteus mirabilis ATCC 25933

Inoculum (cfu) 10-100

Growth : None- poor, Recovery : <=10%, Colony colour : Brown - Black w/o swarming, Lecithinase : (-)

Organism

Escherichia coli ATCC 25922

Inoculum (cfu) 10-100

Growth : None- poor, Recovery : <=10%, Colony colour : Large Brown -Black, Lecithinase : (-)

Organism

Micrococcus luteus ATCC 10240

Inoculum (cfu) 10-100

Growth : Fair - good, Recovery : <=30- 40%, Colony colour : very small Brown- Black, Lecithinase : (-)

Key

Lecithinase halo : (+) indicates halo around colony
Lecithinase halo : (-) indicates no halo around colony

Applications :


Recommended medium for isolation and enumeration of coagulase-positive staphylococci from food and other specimens.

Composition :


Ingredients

gm/lt.

Casein enzymic hydrolysate

10.00

Beef extract

5.00

Yeast extract

1.00

Glycine

12.00

Sodium pyruvate

10.00

Lithium chloride

5.00

Agar

20.00

Sulphamethazine

0.05

Directions :


1. Add 63.05 gm powder to 950.0 ml distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4. Cool to 45-500C.Aseptically add 50 ml egg yolk emulsion and 3 ml of sterile 3.5% Potassium Tellurite solution(99482).
5. Mix well.

Storage

8 to 25°C (Cool & Dry Area)

Shelf Life

36 Months

IMDG Identification

Not Regulated for Transport (Non-Haz)

HSN Code :


100 Gms

38210000 (GST 18%)

500 Gms

38210000 (GST 18%)

Product Datasheet

Sample COA

Certificate Of Analysis