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Light yellow
Free flowing, homogeneous powder
104.40 gm/lit
slightly opalescent
Yellow
7.3 ± 0.2
Clear to very slightly opalescent
Yellow
semi-solid,comparable with 0.4% agar and 8.0% Gelatin
Inoculate and incubate at 35°C for 18-24 hours with the following:
Enterobacter aerogenes ATCC 13048
Growth: Good
Motility : (+)ve
H2S : (-) ve
Urease : (-)ve
Escherichia coli ATCC 25922
Growth: Good
Motility : (+)ve
H2S : (-) ve
Urease : (-)ve
Salmonella typhimurium ATCC 14028
Growth: Good
Motility : (+)ve
H2S : (+) ve
Urease : (-)ve
Urease: -
Staphylococcus aureus ATCC 25923
Growth: Good
Motility : (-)ve
H2S : (-) ve
Urease : (-)ve
Proteus mirabilis* ATCC 25933
Growth: Good
Motility : (±)ve
H2S : (+)ve
Urease : (+)ve
Shigella sonnei ATCC 25931
Growth : Good
Motilit y : ( -)ve
H2S :(-)ve
Urease : (-)ve
Motility : (+)ve, positive reaction, indicated by growth away from stabline
(-)ve, negative reaction, indicated by growth along stab line
* .is temperature dependent, more pronouced at 20°C and absent at 35°C.
H2S : (+)ve, positive reaction, blackening of medium
H2S : (-)ve, negative reaction, no blackening of medium
Urease : (+)ve, positive reaction, dark pink-red colour of medium
Urease : (-)ve, negative reaction, no change in colour of medium
(+)ve: positive reaction, motile organism / blackening of medium/ reddish purple colouration
For the determination of bacterial motility and the ability of bacteria to produce H2S from L- Cystine.
gms/lt.
80.00
10.0
5.00
4.00
3.00
2.00
0.20
0.20
1. Add 104.40 gm powder to 1.0 liter distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Dispense in tubes in 4ml amounts.
4. Autoclave at 10 psi pressure at 115°C for 15 minutes.
5. Allow the tubes to soldify in upright position.
8 to 25°C (Cool & Dry Area)
36 Months
Not Regulated for Transport (Non-Haz)
38210000 (GST 18%)
38210000 (GST 18%)
