Appearance (Colour)
Beige to light beige
Appearance (Form)
Free flowing, homogeneous powder
Solubility
29.00 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
Gel strength
Firm comparable with 1.5% agar gel
pH (25°C)
4.0 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Light amber
Cultural Response
Inoculate and incubate at 45°C for 18-24 hours.
Organism
Alicyclobacillus acidocaldarius ATCC 27009
Inoculum (cfu) 10-100
Growth (30°C) : Good
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth (30°C) : Inhibited
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth (30°C) : Inhibited
Organism
Candida albicans ATCC 10231
Inoculum (cfu) 10-100
Growth (30°C) : Inhibited
Organism
Saccharomyces cerevisiae ATCC 9763
Inoculum (cfu) 10-100
Growth (30°C) : Inhibited
Applications
Used for detection of Alicyclobacillus in fruit juices.
Composition
Ingredients
gm/lt.
Glucose
5.00
Potassium dihydrogen phosphate
3.00
Yeast extract
2.00
Magnesium sulphate
0.50
Calcium chloride
0.25
Copper sulphate
0.00016
Ammonium sulphate
0.20
Cobalt chloride
0.00018
Sodium molybdate
0.00030
Zinc sulfate
0.00018
Mangenese sulfate
0.00015
Boric acid
0.00010
Agar
18.00
Directions
1. Add 29.00 gm to 1.0 liter distilled/purified water and mix throughly.
2. Gently heat and bring to boil to dissolve medium completely.
3. Autoclave at 15 psi at 121°C for 15 minutes.
Note : The media is manufactured with pH 5.3±0.2 to maintain gel strength during sterilization. Adjust the pH at 4.0±0.2 after autoclaving, by adding 1N H2SO4 OR 1N NaOH.
