Appearance (Colour)
Cream to yellow, with pink tint
Appearance (Form)
Free flowing, homogeneous powder
Solubility
44.86 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
Gel strength
Firm, comparable with 1.2% agar gel
pH (25°C)
7.2 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Red
Cultural Response
Inoculate and incubate at 37 ± 2°C for 18-24 hours
Organism
Aeromonas hydrophila ATCC 7966
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Colony characteristics : Yellow, surrounded by yellow zone
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Colony characteristics : Red violet surrounded by red violet zone.
Organism
Pseudomonas aeruginosa ATCC 9027
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Colony characteristics : Red violet surrounded by red violet zone.
Organism
Escherichia coli ATCC 25922
Inoculum
103
Growth : Poor - Fair
Organism
Staphylococcus aureus
ATCC 25923
Inoculum
103
Growth : Inhibited
Applications
Used for detection of Aeromonas and Pseudomonas in food, as well as in equipments and water supplies of food industry.
Composition
Ingredients
gms/lt.
Sodium L-glutamate
10.00
Starch, soluble
20.00
Potassium dihydrogen phosphate
2.00
Magnesium sulfate
0.50
Phenol Red
0.36
Agar
12.00
Directions
1. Add 44.86 gm powder to 1.0 liter distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling to dissolve medium completely.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4. Cool to 45 - 50°C.
5. If desired, add 10,000 IU Peniciilin G sodium salt, 0.01 gm Pimaricin, Mix well.
