1.Add 21.0 gm powder to distilled/deionized water.
2.Bring volume to 1.0 liter and mix thoroughly.
3.Gently heat and bring to boiling.
4.Distribute into tubes.
5.Autoclave at 15 psi pressure at 121°C for 15 minutes.
For the differentiation of aerobic and facultative Gram-negative organisms based on their ability to reduce nitrate.
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