Appearance (Colour)
Cream to yellow
Appearance (Form)
Free flowing, homogeneous powder
Solubility
61.52 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
Gel strength
Firm, comparable with 1.5% agar gel
pH (25°C)
7.0 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent gel with bluish tinge
Prepared Medium Appearance after autoclaving (Colour)
Medium amber
Cultural Response
Inoculate and incubate anaerobically at 37°C for 40 - 48 hours (with added Gen Selective Supplement I).
Organism
Bacteroides fragilis ATCC 23745
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Esculin hydrolysis : positive reaction (blackening of medium)
Organism
Bacteroides vulgatus ATCC 8482
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Esculin hydrolysis : negative reaction, (no blackening of medium)
Organism
Proteus mirabilis ATCC 12453
Inoculum (cfu) 10-100
Growth : None-poor
Esculin hydrolysis : negative reaction (no blackening of medium))
Organism
Clostridium perfringens ATCC 13124
Inoculum
Growth : Inhibited
Applications
Used for the selective isolation, identification and cultivation of Bacteroides fragilis group.
Composition
Ingredients
gms/lt.
Tryptone
15.00
Soya peptone
5.00
Sodium chloride
5.00
Bile
20.00
Esculin
1.00
Ferric ammonium citrate
0.50
Hemin
0.01
Vitamin K1
0.01
Agar
15.00
Directions
1. Add 61.52 gm powder to 1.0 liter distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4. Cool to 45-50°C and aseptically add rehydrated contents of two vials of Gen Selective Supplement I. Mix well and pour in petriplates.
