Appearance (Form)
Liquid
Appearance (Colour)
Colourless
Appearance (Clarity)
Clear
Activity
One unit is defined as the amount of enzyme required to digest 1 µg of lambda DNA in 1 hour at 37 °C in 50 µL of assay buffer
DNA degradation
Incubation of single stranded and double stranded radiolabeled oligonucleotides with 10 units of the enzyme for 4 hours at 37 °C
Star Acitvity
After 160-fold overdigestion (10 U/µg Lambda DNA for 16 hours at 37 °C) with enzyme the fragmentation pattern is analysed. No detectable changes compared to the theoretical fragmentation pattern are considered as absence of star activity
Description
XbaI is an endonuclease enzyme isolated from strains of Xanthomonas badrii, and is part of the restriction modification system.
In molecular biology it is used as a restriction enzyme. It creates sticky ends with 3' end overhangs. The nucleic acid sequence where the enzyme cuts is TCTAGA, which, as the complementary sequence is AGATCT, has rotational symmetry.
Includes
750U
• XbaI - 75μl
• 10X Reaction Buffer - 1.0ml
1250U
• XbaI - 125 μl
• 10X Reaction Buffer - 1.0ml
