Appearance (Form)
Liquid
Appearance (Colour)
Colourless
Appearance (Clarity)
Clear
Concentration
5U/µl
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid insoluble material in 30 minutes at 75°C
Amplification capability
Up to 5kb DNA fragment
Absence of nucleases
Confirmed
DNA contamination
No Genomic or Plasmid DNA contamination
Description
Taq DNA Polymerase is a thermostable recombinant DNA polymerase derived from thermophilic bacterium Thermus aquaticus. Its molecular weight is 94 kDa. Taq DNA Polymerase can amplify DNA target up to 5 kb (simple template). The elongation velocity is 0.9~1.2kb/min (70~75°C). It has 5' to 3' polymerase activity but lacks of 3' to 5' exonuclease activity that results in a 3'-dA overhangs PCR product.
Applications
PCR amplification of DNA fragments as long as 5 kb
DNA labeling.
DNA sequencing.
PCR for cloning.
Includes
500 units
• TAQ Polymerase (5U/μl) - 100 μl
• 10X PCR Buffer (MgCl2 free) - 1.25ml
• 25mM MgCl2 - 1.0ml
1000 units
• TAQ Polymerase (5U/μl) - 200 μl
• 10X PCR Buffer (MgCl2 free) - 1.25mlx2
• 25mM MgCl2 - 1.0ml
5000 units
• TAQ Polymerase (5U/μl) - 200 μlx5
• 10X PCR Buffer (MgCl2 free) - 1.25mlx10
• 25mM MgCl2 - 1.0mlx5
