Appearance (Form)
Liquid
Appearance (Colour)
Colourless
Appearance (Clarity)
Clear
Activity
One unit is defined as the amount of enzyme required to digest 1 µg of lambda DNA in 1 hour at 37 °C in 50 µL of assay buffer.
DNA degradation
Incubation of single stranded and double stranded radiolabeled oligonucleotides with 10 units of the enzyme for 4 hours at 37 °C.
Star Acitvity
After 160-fold overdigestion (10 U/µg Lambda DNA for 16 hours at 37 °C) with enzyme the fragmentation pattern is analysed. No detectable changes compared to the theoretical fragmentation pattern are considered as absence of star activity.
Description
MspCI is an endonuclease enzyme isolated from strains of Micrococcus species and is part of the restriction modification system. It is an isoschizomer of AflII.
In molecular biology it is used as a restriction enzyme. It creates sticky ends with a 3' overhang. The nucleic acid sequence where the enzyme cuts is CTTAAG, which, as the complementary sequence is GAATTC, has rotational symmetry.
Includes
• MspCI - 25 μl
• 10X Reaction Buffer - 1.0ml
