Appearance (Form)
Liquid
Appearance (Colour)
Blue
Electrophoresis test
Dye in 1X concentration is mixed with Plasmid DNA and the mobility is tested by agarose gel electrophoresis.
Stability
It should be stable when subjected to repeated freeze-thaw
Description
6X Blue Loading Dye is a pre-mixed loading buffer with a single tracking dye for agarose and non-denaturing poylacrylamide gel electrophoresis. EDTA is included to chelate magnesium (up to 10 mM) in enzymatic reactions, thereby stopping the reaction. Bromophenol Blue is the standard tracking dye for electrophoresis.
Application
Analysis of large DNA molecules.
Preparation of DNA ladders, markers and samples for loading on agarose or polyacrylamide gels
Features:
Single colour tracking of DNA migration during electrophoresis. No DNA masking during gel exposure to UV light. EDTA binds divalent metal ions and inhibits metal dependent nucleases.
