Appearance (Colour)
Beige to light green
Appearance (Form)
Free flowing, homogeneous powder
Solubility
29.18 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
Gel strength
Firm, comparable with 1.5 % gel
pH (25°C)
6.7 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Emerald green
Cultural Response
Inoculate and incubate at 25 - 30°C for 1-7 days
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good, Acetate utilization : (+)
Organism
Klebsiella pneumoniae ATCC 13883
Inoculum (cfu) 10-100
Growth : Good, Acetate utilization : ( +)
Organism
Proteus vulgaris ATCC 13315
Inoculum (cfu) 10-100
Growth : Inhibited, Acetate utilization : (-)
Organism
Shigella sonnei ATCC 25931
Inoculum (cfu) 10-100
Growth : Inhibited, Acetate utilization : (-)
Applications
For the differentiation of Shigella species from those of the Escherichia genus.
Composition
Ingredients
gm/lt.
Sodium acetate
2.00
Magnesium sulfate
0.10
Sodium chloride
5.00
Monoammonium phosphate
1.00
Dipotassium phosphate
1.00
Bromothymol Blue
0.08
Agar
20.00
Directions
1. Add 29.18 gm powder to 1.0 liter distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Dispense into test tubes.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
5. Allow the tubes to cool in a slanted position.
