Antibiotic Sensitivity (Positive)
DH5α Competent cells, 10µl were streaked into a LB Agar plate containing Nalidixic acid, will form colonies after incubation for overnight at 37°C.
Antibiotic Sensitivity (Negative)
DH5α Competent cells, 10µl were streaked into a LB Agar plate containing Ampicillin, will not form colonies after incubation for overnight at 37°C.
Blue - White Screening
DH5α Competent cells were shown to be suitable for blue white screening by α - complementation of the β - galactosidase gene using pUC19.
Transformation Efficiency
DH5α Competent cells, 50 µl were transformed with 200pg of pUC19 DNA using standard protocol. Incubation overnight on LB Agar plates shown >=1x 106 cfu/µg of DNA.
Description
DH5α Competent Cells are ideal cells that are made efficient chemically for transformation techniques. They are recommended for routine subcloning into plasmid vectors giving a transformation efficiency of ≥1 × 10â¶cfu/μg. Subcloning efficiency cells are not suitable for the generation of cDNA libraries. The φ80dlacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors to allow blue/white colony screening on bacterial agar plates containing X-Gal and similar chemicals.
Includes
2x 0.1ml
DH5α Competent cells - 2 X 0.1ml
pUC19 (10ng/μl) - 50 μl
4x 0.5ml
DH5α Competent cells - 4 X 0.5ml
pUC19 (10ng/μl) - 100 μl
