Appearance (Colour)
Light cream
Solubility
28.00 gm/lit
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
Gel strength
Firm, comparable with 1.5% Agar gel
pH (25°C)
7.4 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Light amber
Cultural Response: Inoculate and incubate at 37° C for 18-24hrs
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth - Good, Recovery Rate >=70%
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth - Good, Recovery Rate >=70%
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Growth- Good, Recovery Rate >=70%
Organism
Streptococcus pyogenes ATCC 19615
Inoculum (cfu) 10-100
Growth - Good, Recovery Rate >=70%
Organism
Salmonella typhimurium ATCC 6539
Inoculum (cfu) 10-100
Growth - Good, Recovery Rate >=70%
Organism
Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100
Growth - Good, Recovery Rate >= 70%
Applications
For the cultivation and maintenance of a wide variety of microorganisms.
Composition
Ingredients
gm/lt.
Agar
15.00
Peptic digest of animal tissue
5.00
Sodium chloride
5.00
Yeast extract
2.00
Beef extract
1.00
Directions
1. Add 28.00 gm powder to 1.0 litre distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4. Cool to 45-50°C.
5. If desired, aseptically add sterile 5-10% defibrinated blood.
