Appearance (Form)
Liquid
Appearance (Colour)
Dark Blue
Electrophoresis test
Dye in 1X Concentration is mixed with Plasmid DNA and the mobility is tested by agarose gel electrophoresis
Stability
It should be stable when subjected to repeated freeze-thaw
Description
6X Tri-colour Loading Dye is a pre-mixed loading buffer with three tracking dyes for agarose and non-denaturing poylacrylamide gel electrophoresis. EDTA is included to chelate magnesium (up to 10 mM) in enzymatic reactions, thereby stopping the reaction. Bromophenol Blue, xylene cyanol and ornageG are the standard tracking dyes for electrophoresis.
Application
Analysis of large DNA molecules.
Preparation of DNA ladders, markers and samples for loading on agarose or polyacrylamide gels
Features:
Tri-color tracking of DNA migration during electrophoresis. No DNA masking during gel exposure to UV light. EDTA binds divalent metal ions and inhibits metal dependent nucleases.
