Appearance (Colour)
Light cream
Appearance (Form)
Free flowing, homogeneous powder
Solubility
13.00 gm/liter
Solubility after autoclaving (Clarity)
Clear without any precipitate
pH (25°C)
7.4 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear without any precipitate
Prepared Medium Appearance before autoclaving (Colour)
Light amber
Cultural Response
Inoculate and incubate at 37°C for 18 - 24 hours
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Growth : Good
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good
Organism
Streptococcus pyogenes ATCC 19615
Inoculum (cfu) 10-100
Growth : Good
Applications
For the cultivation and maintenance of a wide variety of microorganisms.
Composition
Ingredients
gm/lt.
Peptone
5.00
Sodium chloride
5.00
Yeast extract
2.00
Beef extract(ex.buffalo)
1.00
Directions
1. Add 13.00gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15psi pressure at 121°C for 15 minutes.
