Appearance (Colour)
Light yellow to light tan
Appearance (Form)
Free flowing, homogeneous powder
Solubility
56.30 gm/liter
Solubility before boiling (clarity)
Clear to slightly opalescent
Gel strength
Firm comparable with 1.5% agar gel
pH (25°C)
8.0 ± 0.2
Prepared medium appearance after boiling (clarity)
Clear to very slightly opalescent
Prepared medium appearance after boiling (colour)
Dark green
Cultural Response
Inoculate and incubate plates at 37°C for 18-24 hours
Organism
Aeromonas hydrophila ATCC 7966
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Colony colour - dark green, opaque with dark center
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=50.0%
Colony colour- blue/grey, transcluent, pinpoint
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Inhibited
Organism
Salmonella typhi ATCC 6539
Inoculum (cfu) 10-100
Growth : Inhibited
Applications
For the selective differentiation and isolation of Aeromonas hydrophila and other Aeromonas species from clinical specimens and foods.
Composition
Ingredients
gm/lt.
Peptone, special
5.00
Yeast extract
3.00
L-Lysine hydrochloride
3.50
L-Arginine hydrochloride
2.00
Inositol
2.50
Lactose
1.50
Sorbitol
3.00
Xylose
3.75
Bile salts
3.00
Sodium thiosulphate
10.67
Sodium chloride
5.00
Ferric ammonium citrate
0.80
Bromothymol blue
0.04
Thymol blue
0.04
Agar
12.50
Directions
1. Add 28.15 gm powder to distilled/purified water.
2. Bring volume to 500.0 ml and mix thoroughly.
3. Gently heat and bring to boiling.
4. Do not autoclave.Do not Overheat.
5. Cool to 45-50°C.
6. Aseptically add rehydrated contents of 1 vial of Aeromonas Selective Supplement (63013).
7. Mix well and dispense as desired.
