Appearance (Colour)
Beige to tan
Appearance (Form)
Free flowing, homogeneous powder
Solubility
29.00 gm/liter
Solubility before autoclaving (Clarity)
Clear to very slightly opalescent may have slight precipitate
pH (25°C)
7.1 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent may have slight precipitate
Prepared Medium Appearance after autoclaving (Colour)
Light amber
Cultural Response
Inoculate and incubate at 30-35°C for upto 24 - 72 hours
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good
Organism
Clostridium sporogenes ATCC 11437
Inoculum (cfu) 10-100
Growth : Good
Organism
Bacillus subtilis ATCC 6633
Inoculum (cfu) 10-100
Growth : Good
Organism
Bacteroides vulgatus ATCC 8482
Inoculum (cfu) 10-100
Growth : Good
Organism
Candida albicans ATCC 10231
Inoculum (cfu) 10-100
Growth : Good
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Organism
Streptococcus pyogenes ATCC 19615
Inoculum (cfu) 10-100
Growth : Good
Applications
For the sterility testing of biological products which are turbid or viscous as mentioned in pharmacopeia.
Composition
Ingredients
gm/lt.
Yeast extract
5.00
Enzymatic hydrolysate of casein
15.00
Dextrose
5.50
Sodium chloride
2.50
L-Cystine
0.50
Sodium thioglycollate
0.50
Directions
1. Add 29.00 gm powder to 1.0 liter distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Dispense into test tubes.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
