Appearance (Colour)
Cream to yellow
Appearance (Form)
Free flowing, homogeneous powder
Solubility
35.20 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
Gel strength
Firm, comparable with 1.5% agar gel
pH (25°C)
7.2 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Light to medium amber
Cultural Response
Inoculate and incubate at 37°C for 18-24 hours
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good
Organism
Streptococcus pyogenes ATCC 19615
Inoculum (cfu) 10-100
Growth : Good
Organism
Clostridium perfringens ATCC 12919
Inoculum (cfu) 10-100
Growth : Good.
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Applications
For growth of aerobic, anaerobic and microaerophilic organisms. Recommended for sterility testing of solutions and materials not containing mercurial preservatives.
Composition
Ingredients
gm/lt.
Agar
1.00
Proteose Peptone no.3
20.00
Yeast extract
3.00
Glucose
5.00
Beef extract
3.00
Malt extract
3.00
Ascorbic acid
0.20
Directions
1. Add 35.20 gm powder to 1.0 liter distilled/purified water and mix thoroughly
2. Gently heat and bring to boiling.
3. Dispense into test tubes.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
