Appearance (Colour)
Pinkish beige
Appearance (Form)
Free flowing, homogeneous powder
Solubility
35.01 gm/liter
Solubility before autoclaving (Clarity)
Clear without any precipitate
pH (25°C)
7.3 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear without any precipitate
Prepared Medium Appearance after autoclaving (Colour)
Purple
Cultural Response
Inoculate and incubate at 37°C for 18 - 24 hours
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Gas : (+)ve
Acid : (+)ve
Organism
Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100
Growth : Good
Gas : (+)ve
Acid : (+)ve
Organism
Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100
Growth : Good
Gas : (-)ve
Acid : (-)ve
Organism
Staphylococcus aureus ATCC 25923
Inoculum
Growth : Inhibited
Applications
Used for the identification of coliforms in pharmaceutical testing according to harmonized methods.
Composition
Ingredients
gm/lt.
Pancreatic digest of gelatin
20.00
Lactose
10.00
Bile salts
5.00
Bromocresol Purple
0.01
Directions
1. Add 35.01 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Dispense into tubes containing inverted Durham’s tubes.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
