Appearance (Colour)
Beige
Appearance (Form)
Free flowing, homogeneous powder
Solubility
29.00 gm/liter
Solubility before autoclaving (Clarity)
Clear w/o any precipitate
pH (25°C)
7.1 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear w/o any precipitate
Sterility (Bacterial count)
No bacterial and fungal growth is observed after 14 days at 37°C.
Prepared Medium Appearance after autoclaving (Colour)
Light to medium amber
Cultural Response
Inoculate and incubate at 30° - 35°C for not more than 3 days
Organism
Clostridium sporogenes ATCC 19404
Inoculum (cfu) 10-100
Growth : Good
Organism
Bacteriodes vulgatus ATCC 8482
Inoculum (cfu) 10-100
Growth : Good
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good
Organism
Staphylococcus aureus ATCC 6538 P
Inoculum (cfu) 10-100
Growth : Good
Organism
Psuedomonas aeruginosa ATCC 9027
Inoculum (cfu) 10-100
Growth : Good
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Organism
Escherichia coli ATCC 8739
Inoculum (cfu) 10-100
Growth : Good
Organism
Clostridium prefringens ATCC 13124
Inoculum (cfu) 10-100
Growth : Good
Organism
Bacteriodes fragilis ATCC 23745
Inoculum (cfu) 10-100
Growth : Good
Applications
Gamma irradiated sterile powder used for sterility testing of biological products which are turbid or viscous.
Composition
Ingredients
gm/lt.
Casein enzymic hydrolysate
15.00
Yeast extract
5.00
Sodium chloride
2.50
L-Cystine
0.50
Sodium thioglycollate
0.50
Directions
1. Add 29.00gm powder to 1000 ml distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Do not autoclave and overheat.
