Appearance (Colour)
Light cream
Appearance (Form)
Slightly lumpy, homogeneous powder
Solubility
31.50 gm/liter
Solubility before autoclaving (Clarity)
Opalescent
pH (25°C)
6.9 ± 0.1
Prepared Medium Appearance after autoclaving (Clarity)
Opalescent, immediately after autoclaving, becomes clear. On cooling to room temperature, may have presence of slight locculent precipitate
Prepared Medium Appearance after autoclaving (Colour)
Light amber
Cultural Response
Water testing by MPN Method using A1 Broth. Inoculate and incubate at 37°C for 3 hours with the following. Transfer the tubes to 44.5°C water bath for 21+2 hours
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Recovery at 35°C : Good w/gas
Recovery at 44.5°C : Good w/gas
Organism
Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100
Recovery at 35°C : Good
Recovery at 44.5°C : Poor to good
Organism
Bacillus subtilis ATCC 6633
Inoculum
Recovery at 35°C : Inhibited
Recovery at 44.5°C : Inhibited
Applications
For the detection of faecal coliforms in foods, treated wastewater and seawater by Most Probable Number (MPN) Method.
Composition
Ingredients
gm/lt.
Pancreatic digest of casein
20.00
Sodium chloride
5.00
Lactose
5.00
Salicin
0.50
Triton X-100
1.00 ml
Directions
1. Add 31.50 gm powder to1.0 litre distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Dispense into test tubes containing inverted Durham’s tubes.
4. Autoclave at 15 psi pressure at 121°C for 10 minutes
