Appearance (Colour)
Pinkish beige
Appearance (Form)
Free flowing, homogeneous powder
Solubility
50.03 gm/liter
Solubility before autoclaving (Clarity)
Very slightly opalescent
Gel strength
Firm, comparable with 1.5% agar gel
pH (25°C)
7.1 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Reddish purple
Cultural Response
Inoculate and incubate at 37°C for 18 - 24 hours
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Recovery rate : >=50.0%
Colony colour : pink with bile precipitate
Organism
Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100
Growth : Good
Recovery rate : >=50.0%
Colony colour : pink to red
Organism
Klebsiella pneumoniae ATCC 13883
Inoculum (cfu) 10-100
Growth : Good
Recovery rate : >=50.0%
Colony colour : pink, mucoid
Organism
Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100
Growth : Good
Recovery rate : >=50.0%
Colony colour : colourless
Organism
Shigella flexneri ATCC 12022
Inoculum (cfu) 10-100
Growth : Good
Recovery rate : >=40.0%
Colony colour : colourless
Organism
Proteus mirabilis ATCC 12453
Inoculum (cfu) 10-100
Growth : Good
Recovery rate : >=50.0%
Colony colour : colourless
Organism
Salmonella typhi ATCC 6539
Inoculum (cfu) 10-100
Growth : Good
Recovery rate : >=50.0%
Colony colour : colourless
Organism
Salmonella Paratyphi B
Inoculum (cfu) 10-100
Growth : Good
Recovery rate : >=50.0%
Colony colour : colourless
Organism
Salmonella enteritidis ATCC 13076
Inoculum (cfu) 10-100
Growth : Good
Recovery rate : >=50.0%
Colony colour : colourless
Organism
Staphylococcus aureus ATCC 25923
Inoculum
>=103
Growth : Inhibited
Organism
Enterococcus faecalis ATCC 29212
Inoculum
>=103
Growth : Inhibited
Applications
Used for the selective isolation and differentiation of Escherichia coli and coliform bacteria in pharmaceutical testing according to harmonized methods.
Composition
Ingredients
gm/lt.
Pancreatic digest of gelatin
17.00
Agar
13.50
Lactose
10.00
Sodium chloride
5.00
Bile salts
1.50
Peptones (meat and casein)
3.00
Neutral Red
0.03
Crystal Violet
0.001
Directions
1. Add 50.03 gm powder to 1.0 liter of distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
