Appearance (Colour)
Off white to cream
Appearance (Form)
Free flowing, homogeneous powder
Solubility
16.87 gm/liter
Solubility before autoclaving (Clarity)
Slightly opalescent
Gel strength
Firm, comparable with 1.5% Agar gel
pH (25°C)
7.0 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Off white - Light amber
Cultural Response
Inoculate and incubate at 20 -25°C for 7 days
Organism
Chlorella pyrenoidosa ATCC 50476
Inoculum (cfu) 10-100
Growth : Good
Applications
Used for isolation and cultivation of algae from soil, water and sewage.
Composition
Ingredients
gm/lt.
Sodium nitrate
1.00
Magnesium sulphate
0.513
Calcium chloride
0.058
Ferric chloride
0.003
Dipotassium phosphate
0.25
Ammonium chloride
0.05
Agar
15.00
Directions
1. Add 16.87 powder to 1.0 liter distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
