Appearance (Colour)
Beige
Appearance (Form)
Free flowing, homogeneous powder
Solubility
23.00 gm/liter
Solubility before autoclaving (Clarity)
Slightly opalescent
Gel strength
Firm, comparable with 1.5% agar gel
pH (25°C)
7.5 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Opalescent with oil droplets forms in petri plates
Prepared Medium Appearance after autoclaving (Colour)
Light cream to yellow
Cultural Response
Inoculate and incubate at 35 ± 2°C for 24-48 hours with added Tributyrin supplement (87704)
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good
Lipase activity : (+ve)
Organism
Clostridium sporogenes ATCC 11437
Inoculum (cfu) 10-100
Growth : Good
Lipase activity : (+ve)
Organism
Bacillus subtilis ATCC 6633
Inoculum (cfu) 10-100
Growth : Good
Lipase activity : (+ve)
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Lipase activity : (-ve)
Organism
Clostridium perfringens ATCC 12924
Inoculum (cfu) 10-100
Growth : Good
Lipase activity : (-ve)
Key
(+) clear zones around the colonies
(-) no clear zones around the colonies
Applications
For the cultivation and enumeration of lipolytic microorganisms.
Composition
Ingredients
gm/lt.
Peptic digest of animal tissue
5.00
Yeast extract
3.00
Agar
15.00
Directions
1. Add 23.00 gm powder to 990.0 ml of distilled/purified water and mix thoroughly.
2. Add 1 vial of Tributyrin supplement (87704)
3. Gently heat and bring to boiling.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
5. Cool to 45 - 50°C .
6. Mix well and dispense in glass petri plates.
