Appearance (Colour)
Biege
Appearance (Form)
Free flowing, homogeneous powder
Solubility
35.0 gm/liter
Solubility before autoclaving (Clarity)
Clear to very slightly opalescent
Gel strength
Firm, comparable with 1.5% Agar gel
pH (25°C)
7.2 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Light amber
Cultural Response
Inoculate and incubate at 37°C for 18 - 24 hours
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Organism
Salmonella typhi ATCC 14028
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Applications
APHA, FDA recommended medium for detecting plate counts of microorganisms in food, water and waste water.
Composition
Ingredients
gm/lt.
Agar
15.00
Peptic digest of animal tissue
10.00
Beef extract (ex.buffalo)
5.00
Sodium chloride
5.00
Directions
1. Add 35.00 gm powder to 1.0 liter distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
