Appearance (Colour)
Cream
Appearance (Form)
Free flowing, homogeneous powder
Solubility
39.00 gm/liter
Solubility before autoclaving (Clarity)
Slightly opalescent
Gel strength
Firm, comparable with 1.5% agar gel
pH (25°C)
7.4 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Light to medium amber
Cultural Response
Inoculate and incubate at 37°C for 18 -24 hours
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good
Erythromycin 15 mcg Std Zone :22-30 mm
Observed : 22-30 mm
Organism
Enterococcus faecalis ATCC 29212
Inoculum (cfu) 10-100
Growth : Good
Vancomycin 30 mcg Std. Zone : 17-21 mm
Observed : 17-21 mm
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Growth : Good
Gentamicin 10 mcg Std Zone : 16-21 mm
Observed : 16 -21 mm
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Chloramphenicol 30 mcg :Std.Zone : 21-27 mm
Observed : 21-27 mm
Key
Perform antibiotic sensitivity tests as per the latest CLSI Protocol and Standards.
Applications
For antimicrobial susceptibility testing of a variety of microorganisms. For the cultivation and maintenance of Moraxella osloensis and Neisseria meningitidis.
Composition
Ingredients
gm/lt.
Agar
17.00
Beef infusion from
300.00
Acid hydrolysate of casein
17.50
Starch
1.50
Directions
1. Add 39.00 gm powder to 1.0 liter distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4. Dispense into sterile petri plates.
