Appearance (Colour)
Biege
Appearance (Form)
Free flowing, homogeneous powder
Solubility
9.75 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent with slight precipitate
pH (25°C)
7.3 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to slightly opalescent with slight precipitate
Prepared Medium Appearance after autoclaving (Colour)
Colourless to light amber
Cultural Response
After incubation at 37°C for 24 hours, remove the swabs aseptically and streak on appropriate medium (Chocolate Agar or Tryptone Soya Agar plates) Incubate the plates at 37°C for 18-48 hours. After 48 hours of incubation, all cultures should be viable
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth- Good
Organism
Klebsiella pneumoniae ATCC 13883
Inoculum (cfu) 10-100
Growth- Good
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Growth- Good
Organism
Shigella flexneri ATCC 12022
Inoculum (cfu) 10-100
Growth- Good
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth- Good
Organism
Salmonella typhi ATCC 6539
Inoculum (cfu) 10-100
Growth- Good
Organism
Streptococcus pyogenes ATCC 19615
Inoculum (cfu) 10-100
Growth- Good
Organism
Bacteroides fragilis ATCC 25285
Inoculum (cfu) 10-100
Growth- Good
Organism
Streptococcus pneumoniae ATCC 6305
Inoculum (cfu) 10-100
Growth- Good
Organism
Neisseria meningitidis ATCC 13090
Inoculum (cfu) 10-100
Growth- Good
Organism
Vibrio cholerae ATCC 15748
Inoculum (cfu) 10-100
Growth- Good
Organism
Neisseria gonorrhoeae ATCC 43069
Inoculum (cfu) 10-100
Growth- Good
Organism
Haemophilus influenzae ATCC 10211
Inoculum (cfu) 10-100
Growth- Good
Applications
For the transport of swab specimens to prolong the survival of microorganisms.
Composition
Ingredients
gm/lt.
Sodium chloride
3.00
Potassium chloride
0.20
Calcium chloride
0.10
Magnesium chloride
0.10
Monopotassium phosphate
0.20
Disodium hydrogen phosphate
1.15
Sodium thioglycollate
1.00
Agar
4.00
Directions
1. Add 9.75 gm powder to 1.0 liter distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Dispense into test tubes.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
Test Procedure:
1. Inoculate the sterile swabs with suspension of the organisms.
2. Insert the swabs into the upper one third of the medium depth.
3. Then incubate the medium containing the organisms at room temperature for 18-24 hours.
