Appearance (Colour)
Orangish - pink
Solubility
17.20 gm/liter
Solubility before autoclaving (Clarity)
Clear solution
pH (25°C)
7.0 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Yellowish orange
Prepared Medium Appearance after autoclaving (Colour)
Clear solution
Cultural Response
Inoculate and incubate at 35-37°C for 2-4 days
Organism
Pseudomonas aeruginosa ATCC 9027
Inoculum (cfu) 10-100
Growth : Good
Change of colour to purplish red : Positive
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Growth : Good
Change of colour to purplish red : Positive
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Change of colour to purplish red : Negative
Applications
For the confirmation of Pseudomonas aeruginosa in bottle water.
Composition
Ingredients
gm/lt.
Acetamide
10.00
Sodium chloride
5.00
Dipotassium phosphate
1.39
Monopotassium phosphate
0.73
Magnesium sulphate.7H2O
0.5
Phenol Red
0.012
Directions
1. Add 17.20 gm powder to 1.0 liter distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
