Appearance (Form)
Liquid
Appearance (Colour)
Colourless
Appearance (Clarity)
Clear
pH
7.0 - 7.5
PCR amplification
Used along with other dNTP solutions for amplifying DNA fragments of desired sizes
Absence of nucleases
Incubated at 37°C with test samples overnight
Description
dCTP (2’-deoxycytidine 5’-triphosphate) is supplied as a 100mM solution in sterile, deionized water at pH 7.0 to maintain stability. Prepared from monosodium salts of the free nucleotides.
Application
PCR
Long PCR
RT-PCR
cDNA synthesis
Primer extension
DNA sequencing
DNA labeling