Appearance (Colour)
Light yellow
Appearance (Form)
Free flowing, homogeneous powder
Solubility
20.00 gm/liter
Solubility before autoclaving (Clarity)
Clear
pH (25°C)
7.0 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear w/o any precipitate
Prepared Medium Appearance after autoclaving (Colour)
Light to medium amber
Cultural Response
Inoculate and incubate at 37°C for 18-24 hours.
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good
Organism
Escherichia coli ATCC 23724
Inoculum (cfu) 10-100
Growth : Good
Organism
Escherichia coli ATCC 33526 (K802)
Inoculum (cfu) 10-100
Growth : Good
Applications
Used for maintaining and propagating Escherichia coli in molecular biology procedures.
Composition
Ingredients
gm/lt.
Pancreatic digest of casein
10.00
Yeast extract
5.00
Sodium chloride
5.00
Directions
1. Add 20.00 gm powder to 1.0 litre distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4. If desired, add 10% dextrose solution, Mix well.
5. Dispense into sterile test tubes or as desired.
