Appearance (Colour)
Cream to yellow
Appearance (Form)
Free flowing, homogeneous powder
Solubility
46.64 gm/liter
Solubility before autoclaving (Clarity)
Clear to slightly opalescent
Gel strength
Firm, comparable with 1.5% agar gel
pH (25°C)
7.3 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Light yellow
Cultural Response
Incubate at 35 ± 2°C for 18-24 hours
Organism
Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Pigment production : blue-green
Organism
Pseudomonas aeruginosa ATCC 9027
Inoculum (cfu) 10-100
Growth : Good
Recovery Rate : >=70.0%
Pigment production : blue-green
Applications
For the non selective isolation, cultivation and pigment production of Pseudomonas.
Composition
Ingredients
gm/lt.
Proteose peptone
20.00
Potassium sulphate
10.00
Magnesium chloride, anhydrous
1.64
Agar
15.00
Directions
1. Add 46.64 gm powder to 1.0 liter distilled/purified water, containing 10.0 ml glycerol.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
