Appearance (Colour)
Light cream
Appearance (Form)
Free flowing, homogeneous powder
Solubility
17.50 gm/liter
Solubility before autoclaving (Clarity)
Clear to very slightly opalescent
Gel strength (1.5% gel)
Firm, comparable with 1.5 % gel
pH (25°C)
7.0 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity)
Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour)
Light amber
Cultural Response
Inoculate and incubate at 37°C for 18-24 hours
Organism
Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100
Growth : Good,Recovery Rate : >=70%
Organism
Enterococcus faecalis ATCC 29212
Inoculum (cfu) 10-100
Growth : Good,Recovery Rate : >=70%
Organism
Bacillus subtilis ATCC 6633
Inoculum (cfu) 10-100
Growth : Good,Recovery Rate : >=70%
Organism
Streptococcus pyogenes ATCC 19615
Inoculum (cfu) 10-100
Growth : Good,Recovery Rate : >=70%
Organism
Escherichia coli ATCC 25922
Inoculum (cfu) 10-100
Growth : Good,Recovery Rate : >=70%
Organism
Lactobacillus casei ATCC 9595
Inoculum (cfu) 10-100
Growth : Good,Recovery Rate : >=70%
Organism
Lactobacillus acidophilus ATCC 11506
Inoculum (cfu) 10-100
Growth : Good,Recovery Rate : >=70%
Applications
For the enumeration of viable bacteria in milk and dairy products, heterotrophic bacteria in water by pour plate technique.
Composition
Ingredients
gm/lt.
Agar
9.00
Tryptone
5.00
Yeast extract
2.50
Dextrose
1.00
Directions
1. Add 17.50 gm powder to 1.0 liter distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4. Dispense into sterile petri plates.
