Applications :

TE buffer is frequently employed for the storage of DNA, including genomic and plasmid DNA. It can be stored at 4°C for short-term use, while long-term storage is best at temperatures between -20°C and -80°C. To maintain DNA integrity, it's important to avoid repeated freeze-thaw cycles. Beyond storage, TE buffer is useful for breaking protein bonds, which aids in exposing antigens and epitopes in tissue samples preserved with formalin and embedded in paraffin. Additionally, it is beneficial in various protein detection methods, as it enhances antibody staining

Composition :

Description :

TE buffer, often called T10E1 buffer is formulated from two primary components: Tris and EDTA. Tris serves to stabilize the pH, while EDTA protects DNA and RNA by chelating metal ions that are necessary for the activity of degrading enzymes, such as nucleases. This combination effectively preserves the integrity of nucleic acids. For optimal RNA protection, the solution should be maintained at a pH of 7.5, while DNA is best stored at a pH of 8.0. Importantly, a pH of 8.0 is also suitable for storing both DNA and RNA

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