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94837

RT-PCR Kit (First Strand cDNA Synthesis Kit)


Part E
Storage : -20 °C (Blue/Dry Ice)
Shelf Life : 24 Months
HSN Code : 38229090
IMDG Identification :Not Regulated for Transport (Non-Haz)
 
Product Datasheet
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Specifications
 
Appearance (Form)Liquid
Appearance (Colour)Colourless
Absence of nucleasesNo RNase, DNase contamination detected
Notes
  Description:
RT-PCR Kit is optimized to synthesize first-strand cDNA from purified poly (A)+ or total RNA. The RT-PCR kit for RT-PCR delivers increased cDNA yields, high sensitivity, and full-length transcripts in a convenient format. All the components needed for successful first-strand cDNA synthesis is included, saving time and ensuring success with every experiment. The kit Reverse Transcriptase is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability.

The enzyme is used to synthesize cDNA at a
temperature range of 42-55°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases.

Applications:
First strand cDNA synthesis for RT-PCR.
Construction of cDNA libraries.
One-step RT-PCR
Primer extension

Includes:
20 preps
· M-MLV (Recombinant) (200U/μl) - 20.0 μl
· Reaction Buffer (10X) - 40.0 μl
· Oligo (dT) 15 Primer (50μM) - 20.0 μl
· Random Hexamer Primer 6-mer (50μM) - 20.0 μl
· RNasin (40U/ μl) - 12.5 μl
· dNTP Mix (10mM each) - 50.0 μl
· RNase Free Water - 1.0 ml

40 preps
· M-MLV (Recombinant) (200U/μl) - 40.0 μl
· Reaction Buffer (10X) - 80.0 μl
· Oligo (dT) 15 Primer (50μM) - 40.0 μl
· Random Hexamer Primer 6-mer (50μM) - 40.0 μl
· RNasin (40U/ μl) - 25.0 μl
· dNTP Mix (10mM each) - 100.0 μl
· RNase Free Water - 2.0 ml

100 preps
· M-MLV (Recombinant) (200U/μl) - 100.0 μl
· Reaction Buffer (10X) - 200.0 μl
· Oligo (dT) 15 Primer (50μM) - 100.0 μl
· Random Hexamer Primer 6-mer (50μM) - 100.0 μl
· RNasin (40U/ μl) - 60.0 μl
· dNTP Mix (10mM each) - 250.0 μl
· RNase Free Water - 5.0 ml
 
Available Packages
 
Size Price  
20 Preps
INR 7100.00  
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40 Preps
INR 11930.00  
  Add to Cart
100 Preps
INR 28390.00  
  Add to Cart
Prices indicative & subject to additional charges. (Indian Rupees (INR))
 
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